DISASTER MEDICINE No. 4 •2024

https://doi.org/10.33266/2070-1004-2024-4 

EXPERIMENTAL STUDIES

Original article

Application of Paracrine Factors of Stem Cells for Prevention and Treatment of Post-Burn Neovascularization of the Cornea in the Experiment

Tereshchenko A.V. 1,2, Trifanenkova I.G. 1,2, Demyanchenko S.K. 1, Kodunov A.M. 1, Shataev D.A. 1, Iolchiev R.B. 1, Sklifas A.N. 3, Temnov A.A. 3

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1 The Kaluga brunch of the S.Fedorov Eye Microsurgery Federal State Institution, Kaluga, Russian Federation

2 Kaluga State University named after K.E. Tsiolkovskiy, Kaluga, Russian Federation

3 Institute of Cell Biophysics of the Russian Academy of Sciences – a Separate Division of the “Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, Pushchino, Moscow Region, Russian Federation

UDC 617.7-001.17:612.085

Pp. 53-58

 

Summary. Purpose of the study – to evaluate the efficacy of different fractions of a peptide preparation in the treatment of post-burn corneal neovascularization in an experiment.

Materials and methods. The experimental studies were conducted on 40 male Wistar rats. All animals had previously undergone a chemical burn of the cornea and conjunctiva. Various fractions of a peptide preparation containing paracrine factors of mesenchymal stem cells (MSCs) were used as treatment. For the experiment, MSCs were cultivated under hypoxic conditions. Four experimental groups and one control group of 8 rats (8 eyes) were formed. Group 1 – a peptide preparation of the total fraction was used for treatment; group 2 – a peptide preparation with a fraction of molecular weight up to 3 kDa; group 3 – with a fraction of molecular weight up to 30 kDa; group 4 – with a fraction of molecular weight from 30 to 120 kDa, in the control group a culture medium was used as treatment. Before the treatment, as well as on the 14th and 30th day of the experiment, the following studies were performed: photo registration of the fundus, biomicroscopy, corneal OCT, determination of corneal transparency.

Results of the study and their analysis. The study showed that the total fraction and the fraction with a molecular weight of up to 30 kDa, obtained by culturing MSCs under hypoxic conditions, are effective in the treatment and prevention of neovascular corneal leukoma caused by a chemical burn, and these substances may be promising for the development of drugs used in medicine to minimize the effects of injuries in man-made disasters.

Keywords: experimental studies, mesenchymal stem cells, paracrine factors, post-burn corneal neovascularization, prevention, treatment

For citation: Tereshchenko A.V., Trifanenkova I.G., Demyanchenko S.K., Kodunov A.M., Shataev D.A., Iolchiev R.B., Sklifas A.N., Temnov A.A. Application of Paracrine Factors of Stem Cells for Prevention and Treatment of Post-Burn Neovascularization of the Cornea in the Experiment. Meditsina Katastrof = Disaster Medicine. 2024;4:53-58 (In Russ.). https://doi.org/10.33266/2070-1004-2024-4-53-58

 

REFERENCES

  1. Alekseeva G.F., Bordyugova G.G. Features of Diagnostics and Treatment of Burn Contusion Injury. Proceedings of the Scientific-Practical. Conference of Helmholtz Research Institute of City Hospitals. Мoscow Publ., 2001. P. 36-37 (In Russ.).
  2. Akhmedov A.A. Basic Aspects of Vision Injury. Trudy MNIIGB im.Gel’mgol’tsa = Proceedings of Helmholtz Research Institute of City Hospitals. Мoscow Publ., 1998. P.171-172 (In Russ.).
  3. Malyugin B.E., Isabekov R.S., Kalinnikova S.Yu., Antonova O.P. Methods for Diagnosis and Treatment of Corneal Neovascularization. Vestnik Oftal’mologii = Bulletin of Ophthalmology. 2023;139;4: 86-92 (In Russ.). https://doi 10.17116/oftalma202313904186 
  4. Kamilov H.M., Maksudova L.M., Inagamdzhanova Sh.B., Babakhanova D.M. Clinical Assessment of Modern Aspects of the Treatment of Post-Burn Corneal Erosion. Advanced Ophthalmology. 2023;1;1:92-96 (In Russ.). https://doi 10.57231/j.ao.2023.1.1.021
  5. Orazalieva A.M., Ovezklychev P.T., Lamanova D.B., Petrova G.K. The Influence of Autoplasma on Corneal Neovascularization in the Dynamics of Treatment of Purulent Keratitis. Luchshiye Teoreticheskiye i Prikladnyye Issledovaniya 2023: Sbornik Statey Mezhdunarodnogo Nauchno-Issledovatel’skogo Konkursa = Best Theoretical and Applied Research 2023: Collection of articles from the International Research Competition. Penza, Nauka i Prosveshcheniye, 2023. P. 78-81 (In Russ.).
  6. Khazamova A.I., Verigo E.N., Chentsova E.V. The Role of Corneal Microtrauma in the Development of Bacterial Ulcers. Oftal’mologiya = Ophthalmology. 2017;14;2:136–140 (In Russ.).
  7. Samoylov A.S., Astrelina T.A., Aksenenko A.V., Kobzeva I.V., Suchkova Yu.B. Application of Cellular Technologies for Thermal Burn Injuries of the Skin. Saratovskiy Nauchno-Meditsinskiy Zhurnal = Saratov Scientific and Medical Journal. 2019;15;4:999-1004 (In Russ.).
  8. Khubutia M.S., Vagabov A.V., Temnov A.A., Sklifas A.N. Paracrine Mechanisms of Anti-Inflammatory and Organoprotective Action during Mesenchymal Stem Cell Transplantation. Literature Review. Transplantologiya = Transplantology. 2014;1:20-32 (In Russ.).
  9. Khubutiya M.S., Vagabov A.V., Temnov A.A., Sklifas A.N. Paracrine Mechanisms of Proliferative, Anti-Apoptotic and Anti-Inflammatory Effects of Mesenchymal Stromal Cells in Models of Acute Organ Injury. Cytotherapy. 2014;16;5:579-585. https://doi: 10.1016/j.jcyt.2013.07.017
  10. Plakhotniy M.A., Kodunov A.M., Gorina E.V., Boyarintsev V.V., Trofimenko A.V., Biryukov S.A., Filkov G.I. The Influence of Cultivation Conditions of Mesenchymal Stem Cells on their Viability During Transplantation into the Subretinal Space. Biofizika = Biophysics. 2020;65;6:1126-1134 (In Russ.). https://doi 10.31857/S0006302920060125
  11. Wu D., Chan K.E., Lim B.X.H., Lim D.K., Wong W.M., Chai C., Manotosh R., Lim C.H.L. Management of Corneal Neovascularization: Current and Emerging Therapeutic Approaches. Indian J Ophthalmol. 2024;72;3:354-371. https://doi: 10.4103/IJO.IJO_3043_23
  12. Oray M., Abu Samra K., Ebrahimiadib N., Meese H., Foster C.S. Long-Term Side Effects of Glucocorticoids. Expert Opin Drug Saf. 2016;15;4:457-465. https://doi: 10.1517/14740338.2016.1140743.
  13. Tu Y., Luo Y., Zhao Q., Zeng Y., Leng K., Zhu M. Role of Macrophage in Ocular Neovascularization. Heliyon. 2024;10;10: e30840. https://doi:10.1016/j.heliyon.2024.e30840
  14. Apte R.S., Chen D.S., Ferrara N. VEGF in Signaling and Disease: Beyond Discovery and Development. Cell. 2019;176;6:1248–1264. https://doi: 10.1016/j.cell.2019.01.021.
  15. Ahmad A., Nawaz M.I. Molecular Mechanism of VEGF and its Role in Pathological Angiogenesis. J Cell Biochem. 2022;123;12:1938–1965. https://doi: 10.1002/jcb.30344.
  16. Han K.Y., Chang J.H., Lee H., Azar D.T. Proangiogenic Interactions of Vascular Endothelial MMP14 with VEGF Receptor 1 in VEGFA-Mediated Corneal Angiogenesis. Invest Ophthalmol Vis Sci. 2016;57;7:3313–3322. https://doi:doi: 10.1167/iovs.16-19420.
  17. Ebihara N., Matsuda A., Nakamura S., Matsuda H., Murakami A. Role of the IL-6 Classic- and Trans-Signaling Pathways in Corneal Sterile Inflammation and Wound Healing. Invest Ophthalmol Vis Sci. 2011;52;12:8549–8557. https://doi:10.1167/iovs.11-7956.
  18. Ye J., Yao K., Kim J.C. Mesenchymal Stem Cell Transplantation in a Rabbit Corneal Alkali Burn Model: Engraftment and Involvement in Wound Healing. Eye (Lond). 2006;20;4:482-490. https://doi: 10.1038/sj.eye.6701913
  19. Pokrovskaya L.A., Sherstoboev E.Yu., Nadezhdin S.V., Danilets M.G., Trofimova E.S., Ligacheva A.A., Churin A.A., Dubskaya T.Yu Safety of Using a Conditioned Medium Obtained During Targeted Osteogenic Induction of Rat Bone Marrow Mesenchymal Stem Cells. Sibirskiy Nauchnyy Meditsinskiy Zhurnal = Siberian Scientific Medical Journal. 2020;40;2:47–55 (In Russ.). https://doi: 10.15372/SSMJ20200206
  20. Pawitan J.A. Prospect of Stem Cell Conditioned Medium in Regenerative Medicine. BioMed. Res. Int. 2014;2:1–14. https://doi: 10.1155/2014/965849
  21. Sanchooli T., Norouzian M., Ardeshirylajimi A., Ghoreish S.K., Abdollahifar M.A., Nazarian H., Piryaei A. Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats. Iran. Red. Crescent. Med. J. 2017;19: e45516. https://doi: 10.5812/ircmj.45516
  22. Kodunov A.M., Temnov A.A., Tereshchenko A.V., Trifanenkova I.G., Sklifas A.N., Shatskikh A.V. Mechanisms of Influence of the Conditioned Medium of Cultured Stem Cells on the Development of Pathological Angiogenesis of the Cornea in Experiment. Patogenez = Pathogenesis. 2021;19;4:41-52 (In Russ.) https://doi: 10.25557/2310-0435.2021.04.41-52

 

The material was received 11.09.24; the article after peer review procedure 07.10.24; the Editorial Board accepted the article for publication 05.12.24
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